Journal: Stem Cell Research & Therapy

Article Title: A chitosan/acellular matrix-based neural graft carrying mesenchymal stem cells to promote peripheral nerve repair

doi: 10.1186/s13287-024-04093-5

Figure Lengend Snippet: Identification of ADSC. A–D Morphology of ADSC. ADSC exhibited multi-potential differentiation capacity for osteogenesis and adipogenesis. Scale bar: 50 μm, 100 μm, 200 μm. E–H Flow cytometric analysis of cell surface markers of ADSC

Article Snippet: The cell pellet was then centrifuged at 620× g for 10 min, and resuspended in ADSC Dulbecco’s Modified Eagle Medium (DMEM)/F12 (Biochrom) with 100 U/ml penicillin, 100 mg/ml streptomycin (P/S; PAA), 0.2 mM l -ascorbic acid-2-phosphate (A2P; Sigma), and 10% fetal bovine serum (FBS; Biochrom).

Techniques:

Journal: Stem Cell Research & Therapy

Article Title: A chitosan/acellular matrix-based neural graft carrying mesenchymal stem cells to promote peripheral nerve repair

doi: 10.1186/s13287-024-04093-5

Figure Lengend Snippet: Dead/alive staining of acellular nerve microtissue. FDA/PI bichromatic fuorescence staining was performed at 3d, 5d and 7d of ADSC coculture with acellular-MT. Green fluorescence denotes living cells and red fluorescence denotes dead cells. Scale bar: 200 μm

Article Snippet: The cell pellet was then centrifuged at 620× g for 10 min, and resuspended in ADSC Dulbecco’s Modified Eagle Medium (DMEM)/F12 (Biochrom) with 100 U/ml penicillin, 100 mg/ml streptomycin (P/S; PAA), 0.2 mM l -ascorbic acid-2-phosphate (A2P; Sigma), and 10% fetal bovine serum (FBS; Biochrom).

Techniques: Staining, Fluorescence